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Antibiotic Susceptibility Test
Antibiotic Susceptibility Test
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sherwinbrown
12 posts
Jun 22, 2025
11:09 PM
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The disk diffusion method for antibiotic susceptibility testing, also known as the Kirby-Bauer test, is a widely used laboratory technique to evaluate the effectiveness of antibiotics against specific bacterial pathogens. This method helps determine the susceptibility of bacteria to different antimicrobial agents, providing essential information for selecting appropriate treatments in clinical settings.
Here's how the disk diffusion method works:
1. **Preparation**: A pure culture of the bacterial strain is grown and adjusted to a standardized concentration, usually matching the turbidity of a 0.5 McFarland standard. This ensures consistency in the initial concentration of bacteria applied to the test.
2. **Inoculation**: The standardized bacterial suspension is spread evenly across the surface of a Mueller-Hinton agar plate using a sterile swab. This creates a uniform "lawn" of bacteria.
3. **Application of Antibiotic Disks**: Small paper disks impregnated with specific concentrations of various antibiotics are placed on the surface of the agar. Each disk diffuses the antibiotic into the agar, creating a concentration gradient.
4. **Incubation**: The inoculated plates are incubated at 35-37°C for 16-18 hours, allowing bacterial growth and diffusion of the antibiotics through the agar.
5. **Interpretation**: After incubation, the zones of inhibition—clear areas surrounding the antibiotic disks where bacterial growth has been impeded—are measured. The size of these zones is compared to standardized charts to determine whether the bacteria are sensitive, intermediate, or resistant to the antibiotics tested.
The disk diffusion method is valued for its simplicity, cost-effectiveness, and ability to test multiple antibiotics simultaneously. While it provides qualitative results, it remains an essential tool in microbiology laboratories for guiding effective antibiotic therapy and monitoring trends in antibiotic resistance.
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Anonymous
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Jun 23, 2025
5:25 AM
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